Search results for " mesoangioblast"
showing 10 items of 21 documents
Autophagy and apoptosis regolate survival of mesoangioblast stem cells subjected to oxidative stress
2012
Stress response in mesoangioblast stem cells
2006
Stem cells are presumed to survive various stresses, since they are recruited to areas of tissue damage and regeneration, where inflammatory cytokines and cytotoxic cells may result in severe cell injury. We explored the ability of mesoangioblasts to respond to different cell stresses such as heat, heavy metals and osmotic stress, by analyzing heat shock protein (HSP)70 synthesis as a stress indicator. We found that the A6 mesoangioblast stem cells constitutively synthesize HSP70 in a heat shock transcription factor (HSF)-independent way. However, A6 respond to heat shock and cadmium treatment by synthesizing HSP70 over the constitutive expression and this synthesis is HSF1 dependent. The e…
Hsp70 localizes differently from chaperone Hsc70 in mouse mesoangioblasts under physiological growth conditions
2008
Mouse A6 mesoangioblasts express Hsp70 even in the absence of cellular stress. Its expression and its intracellular localization were investigated under normal growth conditions and under hyperthermic stress. Immunofluorescence assays indicated that without any stress a fraction of Hsp70 co-localized with actin microfilaments, in the cell cortex and in the contractile ring of dividing cells, while the Hsc70 chaperone did not. Hsp70 immunoprecipitation assays confirmed that a portion of Hsp70 binds actin. Immunoblot assays showed that both proteins were present in the nucleus. After heat treatment Hsp70 and actin continued to co-localize in the leading edge of A6 cells but not on microfilame…
Hsp70 in mesoangioblast A6 stem cells.
2006
Hsp70 level regulates MMP2 expression in mesoangioblast stemj cells
2013
Intracellular and extracellular Hsp70 in mouse mesoangioblast stem cells
2008
Metalloproteasi nella biologia dei mesoangioblasti di topo
2011
MMP2 synthesis in mouse mesoangioblast stem cells is highly regulated
2012
Autocrine role of extracellular Hsp70 in mesoangioblast migration capability
2014
High-density ZnO Nanowires as a Reversible Myogenic-Differentiation-Switch
2018
Mesoangioblasts are outstanding candidates for stem-cell therapy and are already being explored in clinical trials. However, a crucial challenge in regenerative medicine is the limited availability of undifferentiated myogenic progenitor cells because growth is typically accompanied by differentiation. Here reversible myogenic-differentiation switching during proliferation is achieved by functionalizing the glass substrate with high-density ZnO nanowires (NWs). Specifically, mesoangioblasts grown on ZnO NWs present a spherical viable undifferentiated cell state without lamellopodia formation during the entire observation time (8 days). Consistently, the myosin heavy chain, typically express…